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hydrophobic barrier  (Vector Laboratories)


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    Structured Review

    Vector Laboratories hydrophobic barrier
    Hydrophobic Barrier, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1039 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydrophobic+barrier+pen/ImmEdge+Hydrophobic+Barrier+PAP+Pen/pm42047021-48-1-5
    Average 96 stars, based on 1039 article reviews
    hydrophobic barrier - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Lineage-determining transcription factors constrain cohesin to drive multi-enhancer oncogene regulation
    Article Snippet: A square region was drawn on each coverslip with a hydrophobic barrier pen (Vector Laboratories, cat. no. H-4000) and 200 μl of Granta519 or DND41 cells at 10 7 cells per ml in PBS were gently settled within the barrier.

    Article Title: Protocol to study metastatic dormancy using 4T07-mCherry breast cancer cells and an algorithm-based quantification of disseminated cancer cells
    Article Snippet: Use these settings for all subsequent washing steps in this section. d. Gently dry the region around the sections with a tissue. e. Encircle the dried region of the slide with a hydrophobic barrier pen (Vector Laboratories).

    Article Title: Single-Cell and Spatial Methods for Multimodal Functional Glycan Profiling in Tissues
    Article Snippet: Antigen retrieval was performed at 97°C for 20 min with pH 9.0 Target Retrieval Solution (Agilent, S236784-2) using a PT Module (ThermoFisher, A80400012), after which the slides were cooled to room temperature on the benchtop and washed once in 1× PBS for 5 min. Tissue regions were circled with a hydrophobic barrier pen (Vector Laboratories, H-4000) and then washed in 1× TBS-T for 5 min.

    Article Title: Regional and Temporal Patterns of Long-Term Pseudorabies Virus Detection and Neuropathology in the Murine CNS
    Article Snippet: After air-drying, sections were circumscribed with a hydrophobic barrier pen (Vector Laboratories, H-4000) to confine reagent application to the tissue area.

    Staining:

    Article Title: Obesogenic Inflammatory Memory: A New Concept Related to the Dangerous Effects of Weight Cycling
    Article Snippet: .. A hydrophobic barrier pen (ImmEdge Hydropho-bic Barrier PAP Pen H-4000, Vector Laboratories, Newark, CA, USA) was used to encircle the sections before staining. .. For the evaluation of adipocyte size in adipose tissue, hematoxylin–eosin and Oil red O staining was performed on previously processed and fixed adipose tissue sections for subsequent visualization using a conventional optical microscope (Axioskop model, Zeiss, Oberkochen, Germany).

    Article Title: Obesogenic Inflammatory Memory: A New Concept Related to the Dangerous Effects of Weight Cycling.
    Article Snippet: .. A hydrophobic barrier pen (ImmEdge Hydropho-bic Barrier PAP Pen H-4000, Vector Laboratories, Newark, CA, USA) was used to encircle the sections before staining. .. For the evaluation of adipocyte size in adipose tissue, hematoxylin–eosin and Oil red O staining was performed on previously processed and fixed adipose tissue sections for subsequent visualization using a conventional optical microscope (Axioskop model, Zeiss, Oberkochen, Germany).

    Blocking Assay:

    Article Title: Same-Slide Spatial Multi-Omics Integration with IN-DEPTH Reveals Tumor Virus-Linked Spatial Reorganization of the Tumor Microenvironment
    Article Snippet: The slides were then subject to a series of graded solutions for rehydration using a linear stainer (Leica Biosystems, ST4020), with each step proceeding for 3 min: 3× xylene, 2× 100% EtOH, 2× 95% EtOH, 1× 80% EtOH, 1× 70% EtOH, 3× UltraPure water (Invitrogen 10977- 023), and finally left in UltraPure water (Invitrogen 10977-023). .. Antigen retrieval was then performed at 97°C for 20 min with pH 9 Target Retrieval Solution (Agilent, S236784-2) using a PT Module (ThermoFisher, A80400012), after which the slides were cooled to room temperature on the benchtop and washed in 1× PBS for 5 min. Tissue regions were circled with a hydrophobic barrier pen (Vector Laboratories, H-4000), rinsed in 1× PBS to remove residual ink, then washed in 1× TBS-T prior to photobleaching and antibody blocking. .. For assays that include staining with a biotinylated antibody, an extra biotin blocking step was included at this point with a commercial Biotin Blocking kit (Biolegend, 927301).

    Article Title: H2AK119ub dynamics control hair follicle stem cell quiescence
    Article Snippet: .. Following couple of washes with PBS, the sections were outlined with a hydrophobic barrier pen (ImmEdge PAP Pen, Vector Laboratories, H-4000) and blocked for 2 h at RT or overnight at 4 °C in PBS with blocking solution. ..



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